efficient conjugation of aflatoxin m1 with bovine serum albumin through aflatoxin m1-(o-carboxymethyl) oxime and production of anti-aflatoxin m1 antibodies

Efficient Conjugation of Aflatoxin M1 With Bovine Serum Albumin through Aflatoxin M1-(O-carboxymethyl) Oxime and Production of Anti-aflatoxin M1 Antibodies

BACKGROUND Aflatoxins are the most extensively studied group of mycotoxins produced by molds, especially the Aspergillus group, which are highly toxic to animals and humans. OBJECTIVES Since immunoassay is a simple and rapid method for the analysis of many toxic substances in comparison to the chromatographic methods, it is necessary to produce specific and sensitive antibodies for detection ...

Production and Characterization of Monoclonal Antibodies Against Aflatoxin M1

By using an indirect enzyme-linked immunosorbent assay, four monoclonal antibodies were selected after fusion of mouse P3-NS1-Ag4-1 myeloma cells with spleen cells isolated from BALB/c mice that had been immunized with aflatoxin Ml (AFMI) conjugated to bovine serum albumin. Two of these antibodies were found to be specific for AFMI and were designated AMW-1 and AMW-4. The specificities of AMW-1...

Aflatoxin M1 in Milk - FSA4018

Aflatoxins are mycotoxins of major concern to the dairy industry. Most mycotoxins are found in grain, usually grown in a drought, although they may contaminate pasture grass and occasionally hay. Most frequently, aflatoxins are found in corn (see Figures 1-3) and cottonseed, and sometimes in their byproducts, but also on rare occasions in soybeans or distiller’s grain. Peanut products also may ...

Occurrence of Aflatoxin M1 in Dairy Products

Aflatoxin M1 (AFM1) is a major metabolite of aflatoxin B1 (AFB1), which is formed when animals ingest feed contaminated with aflatoxin B1. The AFB1, once ingested by the animal, is rapidly absorbed by the gastrointestinal tract and is transformed into the metabolite AFM1, which appears in the blood after 15 minutes and is then secreted in the milk by the mammary gland (Van Egmond, 1989; Battaco...

[Aflatoxin M1 in milk and milk products].

Comparative binding and sequence interaction specificities of aflatoxin B1, aflatoxicol, aflatoxin M1, and aflatoxicol M1 with purified DNA.

The covalent binding of the activated forms of several aflatoxins to N-7 of guanine residues on purified DNA has been studied. The aflatoxins include aflatoxin B1 (AFB1) and two human metabolites, aflatoxicol and aflatoxin M1, along with aflatoxicol M1, a rabbit and trout metabolite. DNA binding studies using tritiated [3H]aflatoxins indicate that equimolar solutions of each aflatoxin upon acti...